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BIBLIOGRAPHIC DATA ELEMENTS
1 Title
2 Authors (e.g. creator, co-autors, Laboratory-validation scientist) Name
ID (e.g. ORCID or research ID)
3 Version of the protocol Document dates
Version number (Draft document version number or final document version number)
Documenting substantive changes
4 Provenance of the protocol
DESCRIPTIVE DATA ELEMENTS
5 Overall objective or Purpose
6 Application of the protocol
7 Advantage(s) of the protocol
8 Limitation(s) of the protocol
DATA ELEMENTS FOR THE MATERIALS
9 Sample ID
Strain, genotype or line
Organism name
Sample from organism
Amount of Bio-Source
Developmental stage
Growth substrates
Growth environment
Growing time
Sample pre-treatment or sample preparation
10 Laboratory equipment Laboratory equipment name
manufacturer or vendor name (including homepage)
laboratory equipment ID (catalog number)
Equipment configuration
11 Laboratory consumables or supplies Laboratory consumable name
Manufacturer or vendor name (including homepage)
Laboratory consumable ID (catalog number)
12 Recipes for solutions Solution name
Chemical compound name (ingredient of the solution recipe)
Initial concentration of a chemical compound
Final concentration of chemical compound
Storage conditions
Cautions
Hints
13 Reagents Reagent name
Manufacturer or vendor name (including homepage)
Reagent ID (catalog number)
14 Kits Kit name
Manufacturer or vendor name (including homepage)
Kit ID (catalog number)
15 Software Software name
Software version
Software availability (homepage)
DATA ELEMENTS FOR PROCEDURES
16 Recommendation 1 (list of steps in numerical order)
Recommendation 2 (include alternative steps)
Recommendation 3 (presentation of more that one procedure)
Critical steps
Pause point
Timing
Hints
Troubleshooting
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