Protocols as RDF
From this list there are 5 unpublished protocols; the other 10 are published. We are representing and exposing the content only for published protocols. For unpublished protocols we are only exposing SIRO.
| Protocol | Smart Protocols RDF | SIRO RDF |
|---|---|---|
| [Bio101] Subcutaneous Injection of Tumor Cells | 10.21769_BioProtoc.166.ttl | 10.21769_BioProtoc.166_siro.ttl |
| A simplified universal genomic DNA extraction protocol suitable for PCR |
10.4238_vol10-1gmr1055.ttl | 10.4238_vol10-1gmr1055_siro.ttl |
| Comprehensive analysis of signal transduction in three-dimensional ECM-based tumor cell cultures |
10.14440_jbm.2015.96.ttl | 10.14440_jbm.2015.96_siro.ttl |
| Defining transcribed regions using RNA-seq | 10.1038_nprot.2009.229.ttl | 10.1038_nprot.2009.229_siro.ttl |
| Extraction of total RNA from fresh/frozen tissue (FT) | 10.2144_000113260.ttl | 10.2144_000113260_siro.ttl |
| Isolation of Lung Infiltrating Cell in Mice | 10.21769_BioProtoc.1403.ttl | 10.21769_BioProtoc.1403_siro.ttl |
| Mouse Retinal Whole Mounts and Quantification of Vasculature Protocol | 10.21769_BioProtoc.1546.ttl | 10.21769_BioProtoc.1546_siro.ttl |
| RNA Isolation from Synechocystis | 10.21769_BioProtoc.1428.ttl | 10.21769_BioProtoc.1428_siro.ttl |
| Scratch Wound Healing Assay | 10.21769_BioProtoc.100.ttl | 10.21769_BioProtoc.100_siro.ttl |
| Soft–Agar colony Formation Assay | 10.21769_BioProtoc.220.ttl | 10.21769_BioProtoc.220_siro.ttl |
| Extracción de ADN total (Neoleucinodes - CIAT) | ciat_1_siro.ttl | |
| Total RNA CTAB Extraction Method adapted for Cassava leafs | ciat_2_siro.ttl | |
| Southern,Blotting Protocol for HPT / HPTII gene | Mol-01_siro.ttl | |
| DNA Extraction from rice leaves | Mol-02_siro.ttl | |
| Preparacion de suspension conidial | P-06-6_siro.ttl |
GlycoPOD Protocols as RDF
| Title | GlycoPOD URL | Download RDF |
|---|---|---|
| Quantitative determination of ceramide using human recombinant ceramide kinase | View at GlycoPOD | Download RDF |
| Application of anti-GAG antibody and biotinylated hyaluronan binding protein (bHABP) [1] ~ Detection of hyaluronan using biotinylated hyaluronan binding proteins | View at GlycoPOD | Download RDF |
| Application of anti-GAG antibody and biotinylated hyaluronan binding protein(bHABP) [2] ~ Determination of the concentration of heparan sulfate using ELISA | View at GlycoPOD | Download RDF |
| Conversion of Pyridylamino Sugar Chains to Corresponding Reducing Sugar Chains | View at GlycoPOD | Download RDF |
| Stable isotope labeling of aminosugars with 15N/13C-glucosamine | View at GlycoPOD | Download RDF |
| Permethylation for glycan analysis | View at GlycoPOD | Download RDF |
| Application of anti-GAG antibody and biotinylated hyaluronan binding protein(bHABP) [1] ~ Characteristics and epitopes for anti-heparan sulfate antibodies | View at GlycoPOD | Download RDF |
| Endo-β-N-acetylglucosaminidase H digestion (Endo-H) | View at GlycoPOD | Download RDF |
| Succinylation-Alcian Blue Staining for Mucins | View at GlycoPOD | Download RDF |
| Quantitative determination of ceramide using E. coli diacylglycerol kinase | View at GlycoPOD | Download RDF |
| Application of anti-GAG antibody and biotinylated hyaluronan binding protein(bHABP) [2] ~ ELISA for hyaluronan quantification | View at GlycoPOD | Download RDF |
| Endo-β-N-acetylglucosaminidase F digestion (Endo-F) | View at GlycoPOD | Download RDF |
| Carbohydrate analysis by gas-liquid chromatography | View at GlycoPOD | Download RDF |
| Chemical degradation ~Smith degradation | View at GlycoPOD | Download RDF |
| Endo-β-galactosidase digestion | View at GlycoPOD | Download RDF |
| Rapid and sensitive analysis of O-glycans using an in-line flow glycan-releasing system | View at GlycoPOD | Download RDF |
| Extraction of glycolipids | View at GlycoPOD | Download RDF |
| Large-scale identification of N-glycosylated peptides using lectin-mediated affinity capture, glycosylation site–specific stable isotope tagging, and LC/MS | View at GlycoPOD | Download RDF |
| Glycosidase digestion of O-glycoproteins and related O-glycans | View at GlycoPOD | Download RDF |
| Chemical degradation ~Partial acetolysis | View at GlycoPOD | Download RDF |
| Endo-β-N-acetylglucosaminidase M digestion (Endo-M) | View at GlycoPOD | Download RDF |
| Blue native PAGE | View at GlycoPOD | Download RDF |
| Assay of glucocerebrosidases using HPLC and fluorescent substrates | View at GlycoPOD | Download RDF |
| Endo-β-N-acetylglucosaminidase D digestion (Endo-D) | View at GlycoPOD | Download RDF |
| Serial lectin affinity chromatography | View at GlycoPOD | Download RDF |
| N-Glycan profiling by LC/MS | View at GlycoPOD | Download RDF |
| Preparation of glycoprotein for structural analysis | View at GlycoPOD | Download RDF |
| Simultaneous determination of intracellular nucleotide sugars | View at GlycoPOD | Download RDF |
| Assay of glycosphingolipid synthases using HPLC and fluorescent substrates | View at GlycoPOD | Download RDF |
| Synthesis of sugar oxazolines derived from natural N-glycan as substrates for transglycosylation reaction by Endo-M | View at GlycoPOD | Download RDF |
| Determination of glycan structure by NMR | View at GlycoPOD | Download RDF |
| Thin-layer chromatography (TLC) of glycolipids | View at GlycoPOD | Download RDF |
| Preparation of Membrane Rafts | View at GlycoPOD | Download RDF |
| O-glycosylation analysis by β-elimination in the presence of pyrazolone analogue (BEP) | View at GlycoPOD | Download RDF |
| Quantitative determination of sphingomyelin II | View at GlycoPOD | Download RDF |
| N-Glycanase digestion | View at GlycoPOD | Download RDF |
| Sugar linkage analysis by permethylation (glycosphingolipid) | View at GlycoPOD | Download RDF |
| Specific labeling and chemical analysis of sialic acid and sialyloligo/polymers | View at GlycoPOD | Download RDF |
| Assay of glucocerebrosidases using HPLC and fluorescent substrates | View at GlycoPOD | Download RDF |
| Degradation of Glycolipids by Endoglycoceramidase | View at GlycoPOD | Download RDF |
| Synthesis of glycoproteins in vitro | View at GlycoPOD | Download RDF |
| Detailed analysis of glycosphingolipids at the molecular species level by HILIC/ESI-MS | View at GlycoPOD | Download RDF |
| Simultaneous quantification of glucosylceramide and galactosylceramide by HPLC | View at GlycoPOD | Download RDF |
| TLC-Blot (Far-eastern Blot) and Its Application to MS Analysis of Glycosphingolipids | View at GlycoPOD | Download RDF |
| O-glycan analysis by multi-tandem MS | View at GlycoPOD | Download RDF |
| Hydrazinolysis using hydrazine monohydrate | View at GlycoPOD | Download RDF |